原创论文

为放射治疗而优化的第三代金纳米平台

Published at: 2014年第1卷第19期

Rajiv Kumar 1 , Houari Korideck 2 , Wilfred Ngwa 2 , Ross I. Berbeco 2 , G. Mike Makrigiorgos 2 , Srinivas Sridhar 3
1 Electronic Materials Research Institute and Department of Physics, Northeastern University, Boston, MA, USA; Department of Radiation Oncology, Dana Farber Cancer Institute, Brigham and Women’s Hospital, Harvard Medical School, Boston, MA, USA
2 Department of Radiation Oncology, Dana Farber Cancer Institute, Brigham and Women’s Hospital, Harvard Medical School, Boston, MA, USA
3 lectronic Materials Research Institute and Department of Physics, Northeastern University, Boston, MA, USA; Department of Radiation Oncology, Dana Farber Cancer Institute, Brigham and Women’s Hospital, Harvard Medical School, Boston, MA, USA

摘要

摘要:我们报道的是为放疗应用而优化的基于第三代超微PEG化金纳米粒子平台(AuRadTM)的设计和制备。AuRadTM纳米平台有如下重要特点:(I)表面包被带有胺基、羧基、甲氧基功能基团的异质双功能PEG,以使得该多功能纳米平台可以偶联多种不同的组分,如荧光团、多肽、药物、放射标记物;(II)经优化后的粒子大小适于长时循环、高肿瘤摄取、延缓清除;(III)高辐射增强效应。我们已合成了2-3 nm超微金纳米粒子,再连接上异质双功能PEG,并进一步偶联AlexaFlour 647荧光基团用于光学成像,其稳定性大于6个月。对HeLa细胞共聚焦生物成像,显示出细胞对具有生物相容性纳米粒子的强大摄取能力。X线电离辐射实验显示,集落存活方法证实了细胞杀伤能力增加2.8倍以上。上述结果提示AuRad纳米平台能作为放疗的潜在诊断治疗制剂。

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