原文标题：How do I write a scientific article? A personal perspective
Scientific writing is not an easy task. Although there is no single and universally agreed strategy for assembling a successful scientific article, it is undeniable that some basic notions, gathered after decades of experience, may help increasing the chance of acceptance of a scientific manuscript. Therefore, the purpose of this article is to present a personal and arbitrary perspective on how to write a scientific article, entailing a tentative flowchart and a checklist describing the most important aspects characterizing each section of the manuscript. The final suggestion, which can be summarized in one simple and straightforward concept, is that you should always remember that a scientific article is meant to be read by others (i.e., referees and readers) and not by yourself.
The main drivers of scientific publishing
The goals of scientific or academic writing, which is conventionally defined as a subcategory of publishing aimed at disseminating academic research and scholarship, are many and multifaceted. First of all, the publication of a scientific article is the mainstay for propagating scientific knowledge, by means of a (usually rigorous) peer-review process that is intended to define validity, quality and originality of the study. In the scientists’ perspective, scientific writing is one of the most important criteria for obtaining funds and enabling career progression, since the leading science metrics - Hirsh index (H-index), total impact factor (IF) and number of citations – are based on the number and quality of published articles (1). The capacity to efficiently conveying research findings is hence essential for success in science and medicine (2). More or less consciously, some scientists may also suffer from an obsessive-compulsory (OC) disorder, which makes their life entirely centered around scientific publishing (3). Last but not least, scientific writing contributes to benchmarking (and so defining the ranking of) Universities and other scientific institutions (4).
Although medical and scientific writing has evolved for more than a millennium to present times, the publication of the first ever good practices can be probably dated back to the 14th century (5). Nearly three centuries later, in 1667, the English churchman Thomas Sprat first outlined the foremost concept that literary and scientific writing are not comparable, inasmuch as the latter style should be based on plain, accurate, clear and concise text composition rather than using the rhetorical flourishes characterizing literary language (5). Since then, structuring and writing of medical and scientific information has further evolved in parallel with the progress of science, language and types of media used for delivering scientific information (books, journals, newsletter, e-publishing, websites).
Scientific writing is not an easy task. With the obvious premise that there are no validated or universally agreed criteria set to define how a good scientific article should be assembled, and that each scientist often adopts a personal style to deliver academic research, the purpose of this article is to present a personal and arbitrary perspective on how a scientific article should be written, accumulated after a 25-year experience in scientific writing (6).
According to my personal perspective, a good scientific article should always begin from accurate analysis of experimental data. Therefore, I always start with a comprehensive statistical analysis, occasionally performing statistics that will not be included in the final article for reasons of space or redundancy. As extensively described elsewhere (7), there are several important statistical aspects that should be considered, such as the analysis of values distribution (i.e., Gaussian, non-Gaussian, skewed), the use of appropriate tests (suited to either continuous or categorical values) for establishing differences between measures obtained in different populations or patients’ cohort, the correlation between measured variables (both univariate and multivariate) and - eventually - the impact of measured variables on health risks (i.e., odds or hazard ratios). When the study is centered on diagnostic techniques and/or biomarkers validation, receiver operating characteristics (ROC) curve analysis, diagnostic sensitivity and specificity, negative and positive predictive values, and diagnostic odds ratio should be generally shown. It may also be useful to calculate the “number needed to test” (NNT), i.e., the number of cases that should be measured for identifying one additional adverse outcome. Whenever feasible, diagnostic studies should be arranged around the Standards for Reporting of Diagnostic Accuracy Studies (STARD) guidelines (8,9). Tables and figures may be composed at this stage, bearing in mind that appearance counts. Therefore, they should be self-explanatory, clear and easy to read (i.e., the font size should not be too small).
The “results” section
After statistical analysis has been completed, I usually continue composing the “results” section, along with tables and figures (Fig. 1). This may enable to obtain a clear picture of results and of the entire work, acknowledging the most important findings, the strengths and the potential drawbacks, already at this stage. Do not include excessive or unnecessary data in this section, which may divert attention from the most significant findings. Some journals offer the interesting opportunity to publish supplementary materials. As a rule of thumb, data contained in a table should not be replicated in a figure, and vice versa, and should be presented in objective means. When showing data already present in tables and figures, it may be advisable to use sentences like “a significant difference was found… as shown in the table (or figure)”. The level of statistical significance should always be indicated (e.g., expressions like “p=ns” or “p<0.05” are discouraged), so that the readers can clearly acknowledge the relevance of your findings. Obviously, “p<0.05” can be either p=0.049 or p=49-10, which do not have the same impact. All measures should be given in International System of Units (SI) or SI-derived units, as for journal’s style, and must be absolutely coherent throughout the manuscript (for example, it is extremely annoying, even confusing, to read articles presenting values of the same parameter in “g/L”, “mg/dl”, “mmol/L” throughout the manuscript).
统计分析完成后，我会继续撰写结果部分，同时处理表格和图片（图1）。这样我就可以对结果以及整个工作的有一个全面的了解。在这个阶段，我可以确定研究最重要的发现、优势和潜在的劣势。在这个环节中，不要展示冗余的或者不必要的数据，因为这不利于凸显研究最重要的发现。有些杂志可以上传补充文件。一般来说，表格中已经有了的数据就不应该在图片中再展示，反之亦然。到底是通过表格还是图片来展示数据应该根据自己的目的而定。当描述表格或者图片中已经展示了的数据时，建议使用类似于“a significant difference was found… as shown in the table (or figure)”的句子。统计检验的显著性水平应该标识出来，以便读者能够充分了解你的发现之间的关系，不鼓励使用类似于“p=ns” 或“p<0.05”之类的表述。很明显，P<0.05可能是P=0.049，也可能是P=49-10，对结果影响显然是不同的。所有的测量值都必须用国际单位或者标准单位进行展示，具体要求可以参照杂志的格式要求。文中所有的格式必须统一。如果有一篇文章在展示同一测量时，分别用“g/L”, “mg/dl”和“mmol/L”展示数据，会令读者感觉十分厌烦甚至产生困惑。
Materials and methods
Once results have been written, I usually draft the “materials and methods” section. Although being often underestimated, this is an essential part of the article. The description of the study population, study protocol and methods may help identifying potential weakness in your research, so setting up the remaining parts of the article accordingly. The description of the study population should be comprehensive, thus including sample size, the most important clinical features that have been recorded, as well as potential confounders (i.e. comorbidities, therapies). Do not forget that the ratio between sample size and epidemiologic burden of disease is not meaningless; a study based on measuring glucose in 20 diabetic patients is very unlikely to be published in high IF journals. The description of methods should be exhaustive, so including details about the analytical technique, instrumentation, manufacturers, along with the analytical performance of the assays (e.g., functional sensitivity, imprecision and linearity) (10). This aspect is especially important, because it seldom happens to peer-review articles presenting meaningless values, below the functional sensitivity of the assay, or with differences among patients’ cohorts that are lower than the imprecision of the method. Always remember that, as for ethics in scientific publishing, your article should contain all the necessary information for allowing replication by others. The statistical tests used in the article should be clearly described here, along with details about informed consent and ethical approval, when applicable. Do not present results in this section. A common mistake, for example, is describing here the study population (i.e., using sentences like “the final study population consisted of”), whereas this information must be placed in the “results” section.
结果部分的写作一旦完成，我就开始撰写材料和方法部分。虽然材料和方法部分的撰写经常被人们低估，但是这也是文章的必要部分。对研究人群、研究方案和方法的描述可以帮助人们了解出自己研究的不足，在撰写论文其它部分时也应该考虑到自己研究的不足。对研究对象的描述应该详细，应该包括样本量、已经被记录的一些重要的临床特征、潜在的混杂因素（比如合并症、治疗等）。不要忘了样本量和流行病学发病率之间的比例毫无意义，对于发病率很高的疾病最好是基于大样本的研究。一项基于20个糖尿病患者血糖检测的研究不太可能登上高影响因子的杂志，毕竟糖尿病的发病率很高。对方法学的描述应该详尽，报告的细节应该包括：检测方法、设备、生产商，同时还应该报告方法的分析性能，比如功能灵敏度、不精密度和线性等。这些细节极端重要，因为在经过同行评审的文章中极少出现无意义的结果，比如检测结果比检测方法的功能灵敏度还低，或者不同研究对象之间的差异比方法学的不精密度还低。时刻牢记，你的论文必须包括所有必要的信息，以便其它人能重复你的研究，这是科技出版的规范要求。文中所采用的统计学分析必须描述清楚。如果需要知情同意和伦理学审批，也应交待清楚。在材料和方法中不要展示结果。举个例子，最常见的错误就是在材料和方法中描述研究对象。采用了类似于“the final study population consisted of”之类的句子，这个信息其实应该放在结果部分。
The introduction: the challenge grows
Upon completing the previous two sections (i.e., “results” and “materials and methods”), the challenge moves to “introduction” and “discussion”. Even in such case, there is no one approach better than another, but I usually find more helpful to start with the introduction. In such way, the ensuing discussion of data is often based on a more clear knowledge of the problem you are dealing with. According to my perspective, the introduction should be constructed defining the main aspects of the biological/clinical/laboratory setting, thus including the definition of biological pathways or diseases, the epidemiology, the significance (biological, clinical, social, economic), the frequency of the most likely outcomes (i.e., favorable evolution, recurrence, mortality). The more importance you can deliver on a certain biological or medical aspect, the more likely is to obtain a positive feedback by the referees. Remember also that the readers (and the referees) are not always clinicians, especially if you are willing to publish your article in a translational science or laboratory medicine journal. Therefore, they may somehow struggle to understand the clinical setting, when this is not sufficiently depicted by the authors.
It is not essential, if not unsuitable, to describe previous studies about the same topic in the introduction. The rather spontaneous question that that comes to the mind of the referees when reading an introduction presenting a huge amount of previous data on the same topic is “why have you performed another study, if there is so much already published?”. A reliable approach here, is to generate doubts in whom is reading the manuscript, that previous findings may be contradicted, that the same work can be done better or using a larger sample size, or even that no definitive evidence exists on the topic, so that further work is justified. When supported by data, sentences like “little is known”, “there is still controversy” or “no definitive evidence has been published” may help. Remember to only include material directly related to the topic of your research in this section. It often happens to read papers with very long introductions, that have little to do with the rest of the manuscript. The introduction should be usually concluded with a clear statement about the aim(s) of the study (e.g., “therefore, the aim of this study was…”).
描述前人就同一主题开展的研究并不是必要的。如果前言部分提及了较多的相关研究，审稿人在阅读前言部分时就会自然而然地产生疑问：既然已经有很多相似研究发表了，你为什么还要去开展另一项研究呢？最好的办法就是让论文的读者产生疑问：此前的研究结论并不统一，同样的工作可以做的更好或者可以用更大的样本量进行论证；甚至可以说对于这个问题还没有确定的证据，因此开展进一步的研究是合理的。如果有数据支持，采用类似于“little is known”, “there is still controversy” 或 “no definitive evidence has been published”之类的词句是很有帮助的。记住在前言中，仅仅需要阐述和你研究的科学问题直接相关的内容。如果前言部分写得很冗长，读者常常会在读完前言后对论文的其它部分没啥兴趣。前言部分通常用一个句子来总结研究的目的，比如“therefore, the aim of this study was…”。
Discussion and conclusions
These sections are probably the most important throughout the entire article, since they are meant to deliver the significance of study findings for science, medicine and healthcare in general.
When discussing your findings in this section of the article, raw data should not be repeated, but a remind may be necessary. For example, in the case that a biomarker value in cases and controls was “15 versus 5 mg/L”, it may be advisable to use concise sentences like “the biomarker concentration was found to be 3-fold higher in cases than in controls”, rather than repeating the values.
Data should then be discussed at the light of current knowledge and existing literature. It may be advisable to present here previous findings, after an accurate and comprehensive literature search, so enabling the readers (and the referees) to clearly understand how the actual findings may help improving the knowledge about a specific topic. Irrespective of the possibility that data may be innovative, may contradict or confirm previous findings (bear in mind that the last aspect is often less likely to be well received by the referees), it is always essential to highlight the major strengths setting the work apart from currently available literature. Be very cautious about your conclusions. Biology, and medicine are not exact sciences; it is extremely unpleasant to publish resolute conclusions, that are then contradicted by different findings in ensuing studies. It may hence be advisable to rephrase the final statements with precautious verbs, such as “appear” or “seem”, especially when significance and confidence in your findings are not so strong.
A simple comparison between personal and previously published data may be a good starting point, but is frequently insufficient for article success. For example, if you have discovered a new gene or a new candidate biomarker in the setting of a specific clinical condition or biological pathway, then a tentative pathophysiological explanation should accompany the findings. The simple description of a given phenomenon is always less pervasive than its interpretation according to animal or human biology. Just think at the Scottish biologist Alexander Fleming who casually discovering penicillin in 1928, a finding that has revolutionized medicine. The publication of this “accidental” finding (as literally described by Fleming) in the Bulletin of the World Health Organization in 1929 was not limited to describing that staphylococcal bacteria were dying in proximity to mould colonies contaminating a Petri dish, but he put forward the hypothesis that Pennicillum in culture may produce an antibiotic substance (i.e., penicillin) which could powerfully inhibit the growth of many bacterial species (11). That clear description has made the fortune of Fleming and mankind, paving the way to saving billions lives worldwide. The biological interpretation of data is especially important when findings significantly deviate from those previously obtained by other authors, so that a reliable explanation should be provided for explaining the difference.
The discussion of the study limitations is an open issue. Some journals envisage a specific part of the discussion, usually before the conclusions, where the main drawbacks of the study should be described. In this case, there is not much to choose. Conversely, when this is not mandatory, I personally prefer to partially avert this part, or only add a vague sentence like “further studies are need…”. The potential weakness in the study will likely be captured by the referees and, therefore, the most significant limitations can be introduced upon article revision. I actually consider quite masochistic to submit an article with an entire page of limitations, since this would seems an unconscious appeal to the referees to reject your paper. This is not cheating, this is exactly leaving the referees doing their job.
讨论研究的局限性是比较自由的。有的杂志在讨论部分专门安排了一个环节来讨论局限性，通常放在结论之前。如果是这样，作者没有更多的选择，只能阐述研究的缺陷。如果这不是强制的，我个人更倾向于少写，可以用一些很模糊的句子，比如“further studies are need…”。由于审稿人通常都会指出研究潜在的缺陷，所以可以在修回阶段补充研究最重要的缺陷。事实上，我认为在投稿的文章中用一整页纸来描述研究的局限性是极不明智的。因为这会在不知不觉中导致审稿人拒绝你的稿件。这不是欺诈，准确地说，这是留机会给审稿人做自己的工作。
Although the quality of the process of including citations and references is often underestimated, the sources of information delivered in the text should always be cited. For example, it is unacceptable to read sentences like “it is known that” or “it has been previously demonstrated that” without an accompanying citation. Exactly for the same reason, when the description of a certain biological or clinical aspect is given, this should be associated with evidence that you are not delusional, nor that you are trying to cheat providing untrusty information just to support your findings.
参考文献这个环节经常被很多作者低估。论文中应该标识出学术观点的来源。举个例子：文章中出现了“it is known that”或“it has been previously demonstrated that”之类的句子，但却没有引用参考文献，这就是无法接受的。同样的道理，在描述一个给定的临床或生物学观点时，应该给出相应的证据，证明这一观点并不是自己首创的；否则的话，就认为作者采用了不可信的证据来支持其观点。
The citations to the references in the main body should be prepared exactly as indicated by the instructions to the authors of the journal, and always following the same style. Alternation of citations within round or square brackets, in line with the text or superscripted, is not acceptable. The list of citations should contain the most informative material about the topic. A limited number of self-citations may be helpful to persuade the referees and the readers that you are not really new (or inexpert) about the topic, but a huge number of self-citations should be avoided. Being self-referential is not formally accepted in science and medicine, even if you are a Nobel Prize. Accurately check whether the journal to which you are aiming to submit the article has published similar or overlapping material in recent past. It should be fair (and wise) to add and discuss these articles in your paper and add citations to the references list. The titles of cited journals should be abbreviated as clearly indicated by the instructions to the authors of the journal, and the authors list should also fulfill journal’s criteria.
Preparing a good title is critical, since it should actually reflect finding of the study. Do not use vague sentences such as “analysis of glucose in diabetes”, since this is not expected to capture a substantial interest from referees and readers, overwhelmed by the millions publications available in scientific databases. The use of succinct but clear statements, such as “glucose is increased in diabetics”, is very likely to capture more interest on the article.
准备一个好的标题也是十分重要的。标题应该准确地反映研究的结果。不要使用很模糊的词句，比如“analysis of glucose in diabetes”，因为科学数据库里面的论文多如牛毛，你不要指望这样的标题能激起审稿人和读者的阅读兴趣。应该使用简单名了的句子，比如“glucose is increased in diabetics”，这样的句子作为论文标题更容易受到关注。
The abstract is typically the last part of the manuscript to be written, since it may also be a kind of “collage” of text already written in other parts of the article. It may be advisable to rephrase these parts (e.g., reediting the sentences or changing some words), but the concepts will obviously remain the same. The length and style must be strictly coherent with word count specifications and journal’s instructions. Notably, exceeding the word count almost inevitably results in the manuscript being returned to the authors. Avoid writing abstracts with lengthy “background”, and preferably use the text to focus on results and conclusions. If occasionally happens to read abstracts with 50% of text devoted to the background and only 2-4 lines in which results and conclusion are summarized.
The list of authors
The International Committee of Medical Journals Editors (ICMJE) has established four essential criteria according to which the authorship can be credited. These include (i) substantial contributions to conception or design of the study; or the ac quisition, analysis, or interpretation of data for the study; AND (ii) drafting the article or revising it critically for important intellectual content; AND (iii) finally approving the version of the article to be submitted; AND (iv) agreement to be accountable for all aspects of the study in ensuring that questions related to the accuracy or integrity of any part of the work are appropriately investigated and resolved (12). There is no exemption to these rules, and each author should be aware that any deviation should be interpreted as ethical misconduct. Unfortunately, the real life is often different from the idealized picture. There is a nice story on the Internet, which gives a funny - but seldom realistic – interpretation of who deserves credit, as well as his/her position in the list of authors, within a scientific article. According to this tale, the first author is a senior graduate student who made the figures, the second author is a grant student who has nothing to do with the project but was included because he/she hang around group meetings usually bringing food, the third author is a first year student who actually performed all the experiments, the statistical analysis and wrote the entire article (and still thinks that being third is “fair”), the middle authors are undergraduate students and technical staff, the penultimate author is an ambitious assistant professor who instigated the paper, whereas the last author is the head of the laboratory, who has not even read the paper but… he is the boss, he got the funding and his name is so famous that the article will hardly be rejected. Besides this funny joke, the ICMJE criteria are and will remain for long the cornerstones for establishing who ultimately deserves to be credited in the paper, in the acknowledgements, or not included in the article. Although there are no written rules about the positions in the authors’ list, I have always thought that the scientist who has written the greatest part of (or the entire) article should always be first, the scientist who has thoughtfully revised (and hopefully improved) the article should be last, whereas all the other scientists should be placed in the middle of the author list, according to their actual contribution. Some journals specifically require to list authors’ contributions at the beginning or at the end of the article. This section should hence be prepared ethically and fairly; no credit should be given to authors who have not really contributed to a certain activity.
How should I write?
Writing in a good English, considering that the vast majority of scientific articles are published in this langue, is often an insurmountable barrier for both English native and non-English native speakers, but the challenge is obviously magnified for the latter category of scientists. Even the best work, presenting highly innovative or outstanding findings, may finally be rejected if it cannot be fully understood by the referees. As for other human activities, good writing is always a compromise between innate skills and practice. A quite common custom is to have the article revised by the so-called English “teachers”. This not always works since, as discussed below, scientific and literary languages are not alike, scientific terminology is unique and often carries specific meanings. I have no personal experience on professional bioscience writers, since I have never used their help. Nevertheless, they can offer various services, such as full-text translation or revision, comments about improving the article before submission, selection of the most suitable journal to submit the work, artwork preparation, help for revising or resubmitting the article (13). Some of these services are now also offered by some scientific journals, but a number of shortcomings are apparent. First, all these services do not come for free. Then, professional science writers are not necessarily expert of one certain subject, so that they may misinterpret some key concepts of the article. Notably, the boundaries between professional medical writing, “guestwriting” (writing an article for someone else) and “ghostwriting” (i.e., writing an article but hiring authorship credited to another person) are not so clearly defined, so that some journals are no longer accepting submission prepared by professional science writers (14). Consider also that scientific writers can be legitimate contributors to the article but, according to the criteria of the ICMJE, their contribution must be recognized. Importantly, the long-standing practice of copying and pasting text from other articles is considered as a misconduct (i.e., plagiarism), and can now be easily detected by Editors and publishers using dedicated software programs (15).
As previously discussed, data should not be repeated multiple times throughout results, discussion, conclusion, tables and figures. Redundant information not only increase the production costs for the publishers (each printed pages has a considerable price), but may also antagonize the referees, who may be forced to read the same aspects many (and unnecessary) times. Full results should always be presented once (decided where, among tables, figures or results section).
Another aspect that should be clearly acknowledged is that scientific writing is not like writing a literary piece. The style and construction of sentences may be quite different. When a scientists reads an article, he/she wants to get clear messages, with no flowery prose. Therefore, long, intricate and pompous sentences should always be avoided. Each sentence should have a clearly identifiable subject, a verb and a succinct description of the outcome. It should be no longer than 1-2 lines. It occasionally happens to see papers with very long sentences (i.e., 5-6 lines), so that you no longer remember which is the subject when you get to the end or you need to read the sentence 2-3 times before connecting the subject (usually at the beginning of the sentence) with the verb (at the end of the sentence). The verb should always be placed close to the subject, the “core” of the sentence should be preferably moved to the end, whilst passive verbs should be avoided.
The form, grammar and spelling should be accurately checked (many times, even by different authors) and the use of informal style (e.g., “haven’t” should be “have not”, “wasn’t” should be “was not”, etc.) or slang and colloquial speech should be limited. I do not personally like using characters like “!” or open questions (i.e., placing a question mark [“?”] at the end of a sentence). The use of adverbs and adjectives may be a good means for highlighting, emphasizing or reinforcing important issues. For example, writing “we found a difference” is not the same as writing “we found a considerable difference”, provided that the difference is really noteworthy.
仔细检查格式、语法、拼写，需要多次检查甚至由不同的作者来检查。应该少用一些不正式的写法（比如 “haven’t”应该是“have not”， “wasn’t”应该是“was not”等）或者俚语、口头话。我个人不喜欢用类似于感叹号之类的符号，或者使用反问句（在句末用问号）。用副词或者形容词表示强调和凸显是一种不错的做法。比如，“we found a difference”和“we found a considerable difference”是不同的，后者表示这个差异需要引起关注。
The final layout
The Editor and the referees are often convinced that negligence in the format of the article actually reflects negligence in the research. Their judgment about the quality of the article may hence be unwittingly biased. Therefore, the format should be accurate, in line with the instructions of the authors of the journal. This may also prevent delay in the editorial process, since some journals return the paper to the authors when the instructions are not carefully followed. The type and size of the font is often indicated by the instructions to the authors of the journal. However, when this information is unavailable, a clear font such as “Times New Roman”, “Arial”, “Calibri” or “Book Antiqua” is preferable. Avoid using different fonts, and especially different sizes (usually a 12-point font size is preferable). Use double spacing and margins of 2-3 cm throughout the manuscript. It often happens to receive papers with single spacing and narrow margins, which are very difficult to read.
编辑和审稿人通常认为在格式调整上粗心大意的作者科研工作也不太可能严谨。因此他们对论文质量的判断在不经意间就会发生偏差。因此，论文的格式应该严格按照稿约来进行调整。如果格式不正确会延迟稿件的发表，因为编辑如果发现作者的格式不符合杂志的要求时，就会把稿件返回给作者。应该严格按照稿约中的要求调整字体和字号。如果稿约中未对字体和字号提出要求，建议使用通用的格式如“Times New Roman”, “Arial”, “Calibri”或“Book Antiqua”。避免使用不同的字体和字号（通常建议字号大小为12号）。整篇文章的行距设置为两行，页边距设置为2-3厘米。我经常会收到一些单倍行距，页边距也很窄的稿件，阅读起来很麻烦。
The paper should be actually organized as specifically indicated by the instructions to the authors of the journal. Typically, most journals need that the main text of full-length articles be divided into 5-6 sections, i.e., “introduction”, “materials and methods”, “results”, “discussion” (and/or “conclusions”) and “references”. When different specifications are given (e.g., some journals place the section “materials and methods” at the end of the paper), these should be fulfilled. Carefully avoid to mix up material among the different sections of the article.
As a general rule, the paragraphs should be uniform throughout the article (either aligned or not), and they should all be indented or not indented (according to the instructions to the authors of the journal). The abbreviations should always be spelled. One major issue that challenges the referees is trying to understand what an acronym actually means (e.g., “HF” may stand for “Heart Failure”, but also for “Hydrogen Fluoride”, “Hip Fracture”, “Hemorrhagic Fever”, “High Frequency”, etc.). Tables, figures and supplementary material should accompany the manuscript as indicated by the instructions to the authors of the journal. Therefore, they should not be included in the main document when this is not expressly requested by the journal.
总体原则是，所有的段落必须格式统一，应根据稿约决定各个段落是否对齐，是否缩进。缩写一定要标识出来。很多时候审稿人可能并不知道缩写的意思。比如，HF可以表示Heart Failure(心衰)、Hydrogen Fluoride（氟化氢）、Hip Fracture（髋骨骨折）、Hemorrhagic Fever（出血热）和High Frequency（高频）。根据稿约，表格、图片和补充材料应该在正文中标识出来。他们不需要放在插入正文，除非杂志的稿约中另有说明。
As a general rule, read the article not less than 2-3 times before submission, or having it carefully read by one or more coauthors. In particular, check multiple times the grammar and the style. It is quite annoying to read papers with no space between a full stop or a comma and the following word. I usually have a final pass on the article on separate days, when the mind is fresh. You can read the article for up to 10 consecutive times without findings errors or typos, but it is very likely that you will catch them on another day. Do not rush to submit. One day more will not have such a bad impact on history of science and destiny of your career.
Selecting the journal and submitting the paper
The final and almost unavoidable activities are selecting the most appropriate journal and then submitting the article (16). As for my personal experience, the selection of the target journal can be already made after writing the sections “results” and “materials and methods”, since the information therein contained may be sufficient to identify the audience and should hence guide the composition of the remaining parts of the manuscript. There are at least five main criteria driving the choice of one journal over another: (i) aims and scope of the journal (some journals that have previously published similar material can often be found in your references list), (ii) sample size and significance of the findings, (iii) journal impact (e.g., indexing, IF, visibility, scientific reputation), (vi) policy and costs of publication (open-access; pay for submitting or publishing); and (v) article types (e.g., some journals publish “Brief communications”, “technical brief”, “technical reports”, “research letters” or similar types of papers that are more suited for studies with limited sample size, preliminary or only confirmatory data). The clinical significance of results should not be either oversized or underestimated. This would enable to reduce the overall number of unfavorable revision outcomes and shorten the time to publication. Carefully avoid to submit your article to the so-called “predatory” journals, i.e., financial profit entities based on article processing charges, which do not meet scholarly publishing standards (17). Scientific articles published by predatory publishers will be actually meaningless for your curriculum, since these journals will never be indexed, will never get an IF and, even more importantly, they may suddenly disappear shortly after the publication of your article.
As for the earliest premise, that there is no single and validated approach to write a successful scientific article (18), it is undeniable that some basic notions gathered after years of experience may help increasing the chance of acceptance (Table 1). To put it simply, all the previous concepts can be summarized in one really simple and straightforward concept. A scientific article is meant to be read by others (i.e., referees and readers) and not by yourself. Seems apparently paradoxical, but readers do not just read, but also interpret what they read. These seemingly obvious notions are often overlooked by many scientists, who follow a fairly hedonistic approach in what they write, which may ultimately bother the referees and disappoint the readers.
I really hope that some of the concepts expressed in this dissertation may be a guide or a help, especially for young scientists, who frequently struggle with scientific writing.
Conflicts of Interest: The author has no conflicts of interest to declare.
1. Lippi G, Mattiuzzi C. Scientist impact factor (SIF): a new metric for improving scientists’ evaluation? Ann Transl Med 2017. doi: 10.21037/atm.2017.06.24.
2. Turbek SP, Chock TM, Donahue K, et al. Scientific Writing Made Easy: A Step-by-Step Guide to Undergraduate Writing in the Biological Sciences. Bull Ecol Soc Am 2016;97:417-26.
3. Lippi G, Plebani M, Franchini M. The syndrome of the "obsessive-compulsory scientist": a new mental disorder? Clin Chem Lab Med 2013;51:1575-7.
4. Lippi G, Mattiuzzi C. Scientific publishing in different countries: what simple numbers do not tell. Ann Res Hosp 2017. doi: 10.21037/arh.2017.07.01.
5. Taavitsainen I, Pahta P. Medical and Scientific Writing in Late Medieval English. Cambridge University Press, Cambridge, United Kingdom, 2004. ISBN-13: 978-0521831338.
6. Zhong JS. Living legend in clinical biochemistry-Prof. Giuseppe Lippi: we should have strength to stick to things we do. Ann Transl Med 2016;4:322.
7. Lippi G, Mattiuzzi C. The biomarker paradigm: between diagnostic efficiency and clinical efficacy. Pol Arch Med Wewn 2015;125:282-8.
8. (Lippi G. STARD guidelines: another piece of an intricate puzzle for evaluating the quality of scientific publishing. Ann Transl Med 2016;4:42.
9. Lippi G, Plebani M. Improving accuracy of diagnostic studies in a world with limited resources: a road ahead. Ann Transl Med 2016;4:43.
10. Rifai N, Annesley TM, Berg JP, et al. An appeal to medical journal editors: the need for a full description of laboratory methods and specimen handling in clinical study reports. Clin Chem Lab Med 2012;50:411-3.
11. Fleming A. On the antibacterial action of cultures of a penicillium, with special reference to their use in the isolation of B. influenzae 1929. Bull World Health Organ. 2001;79:780-90.
12. International Committee of Medical Journals Editors. Defining the Role of Authors and Contributors. Available at: http://www.icmje.org/recommendations/browse/roles-and-responsibilities/defining-the-role-of-authors-and-contributors.html. Last access: 15 July, 2017.
13. Das N, Das S. Hiring a professional medical writer: is it equivalent to ghostwriting? Biochem Med (Zagreb) 2014;24:19-24.
14. Reider B. Ghostwriters and Guest-writers. Am J Sports Med 2010;38:2183-5.
15. Masic I, Begic E, Dobraca A. Plagiarism Detection by Online Solutions. Stud Health Technol Inform 2017;238:227-30.
16. Shokraneh F, Ilghami R, Masoomi R, et al. How to select a journal to submit and publish your biomedical paper? Bioimpacts. 2012;2:61-8.
17. Laine C, Winker MA. Identifying predatory or pseudo-journals. Biochem Med (Zagreb) 2017;27:285-91.
18. Weinberger CJ, Evans JA, Allesina S. Ten simple (empirical) rules for writing science. PLoS Comput Biol 2015;11:e1004205.
Checklist describing of the most important aspects characterizing each section of a scientific article
Personal flowchart for writing scientific articles.